At the Seisho Music Academy, the 99th Graduating Class is rehearsing for the annual production of the theatrical play, Starlight. Behind the scenes, however, an underground "Revue Starlight" audition, orchestrated by a talking giraffe, pits the students against each other in stage battles in order to shine as the top star. Karen Aijō, upon being reunited with her childhood friend Hikari Kagura, comes across these auditions and battles to become the top star alongside Hikari.

After the events of Revue Starlight ―The LIVE― #1 revival, Seiran General Arts Institute challenges Seisho Music Academy to a revue with the right to perform the play "Starlight" at stakes. Who will win this Cultural Exchange Program?

As a new school year begins at Seisho Music Academy, nine third-year students in Class A of the Actor Training Department deepen their bonds as they approach graduation. They reminisce about their growth and face uncertain futures while preparing for classes. The story revolves around their determination to pursue their dreams and the challenges they encounter during the Giraffe's Revue, highlighting their growth and transition to the next chapter of their lives.

At Seisho Music Academy's Actor Training Department, which Hikari Kagura has left, the days that Karen, Maya, Junna, Mahiru, Nana, Claudine, Futaba, and Kaoruko will spend together will soon come to an end. With graduation day just around the corner, the career paths that the nine have chosen for after graduation are varied as well. Difficult exams, advanced assignments, new environments, and the chance to show off their own skills... Everyone spends every day with as much anxiety as hope. Who is their true rival? What is it that they truly must fight against?? What awaits them in the Twilight Theater?! The climax is right up ahead. The curtain rises on the best Revue in history!