Set during the declining years of the Tokugawa shogunate, Shadow Hunters details the questionably noble exploits of three ronin who act as "Shadow Hunters". These three ronin are not your normal ornery ruffians who are looking for a drink, a broad and someone to jab a sword into, but are in fact former samurai who, rather than follow their destroyed fiefs and murdered masters into death via seppuku, have dedicated their combined sword prowess to stopping the government from raping its daimyos for valuable resources.

The great Daimyo enlists the aid of the three Shadow Hunters in ensuring the delivery of an illegal cannon in this sequel to Toshio Masuda's bloody adaptation of the manga by Takao Saito. Despite the Shadow Hunters's remarkable skill, this mission is no walk in the park, and in order to survive they must face a relentless army of ninjas and female warriors willing to sacrifice their lives to disrupt the delivery and claim the cannon for themselves.