Having witnessed his parents' brutal murder as a child, millionaire philanthropist Bruce Wayne fights crime in Gotham City disguised as Batman, a costumed hero who strikes fear into the hearts of villains. But when a deformed madman known as 'The Joker' seizes control of Gotham's criminal underworld, Batman must face his most ruthless nemesis ever while protecting both his identity and his love interest, reporter Vicki Vale.

The monstrous Penguin, who dwells in the sewers beneath Gotham, joins up with corrupt mayoral candidate Max Shreck to topple the Batman once and for all. But when Shreck's timid assistant Selina Kyle finds out, and Shreck tries to kill her, she's transformed into the sexy Catwoman. She teams up with the Penguin and Shreck to destroy Batman, but sparks fly unexpectedly when she confronts the caped crusader.

Batman faces off against two foes: the schizophrenic, horribly scarred former District Attorney Harvey Dent, aka Two-Face, and the Riddler, a disgruntled ex-Wayne Enterprises inventor seeking revenge against his former employer by unleashing his brain-sucking weapon on Gotham City's residents. As the caped crusader also copes with tortured memories of his parents' murder, he has a new romance, with psychologist Chase Meridian.

Batman and his sidekick Robin attempt to the foil the sinister schemes of a deranged set of new villains, most notably the melancholy Mr. Freeze, who wants to make Gotham City into an arctic region, and the sultry Poison Ivy, a botanical femme fatale. As the Dynamic Duo contend with these bad guys, a third hero, Batgirl, joins the ranks of the city's crime-fighters.